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Exercise alters susceptibility to LPS‐induced lung injury
Author(s) -
Pastva Amy,
Griffin Michael,
Giamberardino Charles,
Wright Jo Rae
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.955.9
Subject(s) - neutrophilia , lipopolysaccharide , bronchoalveolar lavage , medicine , aerosolization , lung , methacholine , respiratory system , cytokine , immunology , immune system , bronchial hyperresponsiveness , endocrinology , respiratory disease , pathology , inhalation , anesthesia
Lipopolysaccharide (LPS) is an airborne microbiological contaminant that has been associated with adverse respiratory symptoms and asthma exacerbations. Because exercise modulates the immune system, we hypothesized that an active lifestyle attenuates susceptibility to LPS‐induced lung injury. Mice (C57BL/6; 8 wks) were exercised at moderate intensity 5 days/wk for 4 wks for 45 min or were exposed to similar environmental conditions but remained sedentary. After training, mice were challenged with either filtered air (FA) or aerosolized LPS (~4 μg/m 3 ) for 2.5 hrs and measurements were taken 4 and 24 hrs after aerosol exposure. Although LPS treated mice had increased cell counts, neutrophilia, protein levels, and cytokine KC levels in the bronchoalveolar lavage fluid compartment compared to FA mice, similar levels were noted between LPS treated sedentary (LS) and exercised mice (LE). However, in the lung tissue compartment histological analysis revealed that LE mice had decreased perivacular and peribronchial cellular infiltrates compared to LS mice. In preliminary lung function tests, LE mice exhibited reduced airway hyperresponsiveness to aerosolized methacholine challenge compared to LS mice. Taken together, these data suggest that exercise alters the susceptibility of mice to LPS‐induced lung injury.