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Habitual aerobic exercise is associated with reduced vascular endothelial cell oxidative stress, NADPH oxidase and NFκB in middle‐aged and older humans
Author(s) -
Pierce Gary L.,
Donato Anthony J.,
Silver Annemarie E.,
Chiang Rhea,
Seals Douglas R.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.955.18
Subject(s) - nadph oxidase , oxidative stress , medicine , endocrinology , brachial artery , endothelial dysfunction , superoxide dismutase , nitrotyrosine , endothelium , reactive oxygen species , aerobic exercise , chemistry , nitric oxide synthase , nitric oxide , biochemistry , blood pressure
Middle‐aged and older (MA/O) adults who perform habitual aerobic exercise demonstrate enhanced vascular endothelial function compared with their sedentary peers, but the molecular mechanisms involved are unknown. Brachial artery flow‐mediated dilation, a measure of endothelium‐dependent dilation, was 34% higher (6.8 ± 0.9 vs. 5.1 ± 0.4 %δ, P<0.05) in regularly exercising (EX, n=12, 62 ± 2 yrs, 8M/4F) vs. sedentary (SED; n=25, age 62 ± 1 yrs, 12M/13F) healthy MA/O adults, whereas endothelium‐independent dilation to sublingual nitroglycerin was not different (29.3 ± 1.8 vs. 26.7 ± 1.4 %δ, P=0.29). In endothelial cells obtained from the brachial artery, expression of nitrotyrosine (NT), a cellular marker of oxidative stress (0.48 ± 0.07 vs. 0.74 ± 0.10 immunofluorescent intensity/HUVEC intensity), NADPH oxidase p47 phox , a subunit of the oxidant enzyme NADPH oxidase (0.41 ± 0.04 vs. 0.76 ± 0.09), and nuclear factor κB (NFκB), a redox‐sensitive proinflammatory transcription factor (0.39 ± 0.04 vs. 0.64 ± 0.06), were 35‐50% lower in EX, whereas manganese superoxide dismutase (MnSOD), a mitochondrial antioxidant enzyme, was 74% higher (0.48 ± 0.07 vs. 0.28 ± 0.04) (all P<0.05 vs. SED). NT was positively related to NADPH oxidase p47 phox (r=0.64, P<0.01) and NFκB (r=0.29, P=0.05), but not to MnSOD. Compared with their sedentary peers, habitually exercising MA/O adults have reduced vascular endothelial cell oxidative stress related to decreased NADPH oxidase and NFκB. Supported by NIH AG000279, AG013038, AG022241, AG006537, and RR00051.