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Hyaluronidase treatment of the glycocalyx attenuates insulin sensitivity during an intravenous glucose tolerance test in rats
Author(s) -
Eskens Bart,
Cobelens Hanneke,
Vink Hans,
VanTeeffelen Jurgen
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.950.7
Subject(s) - glycocalyx , insulin , hyaluronidase , bolus (digestion) , medicine , saline , chemistry , venous blood , endocrinology , enzyme , biochemistry
The potential of insulin to stimulate glucose uptake from blood to tissues is closely related to the ability of insulin to increase capillary blood volume. The endothelial glycocalyx forms a relatively thick interface between blood and the vascular wall, and recent studies have demonstrated that recruitment of glycocalyx is a powerful tool to effectively increase capillary blood volume. We therefore hypothesized that enzymatic degradation of the glycocalyx impairs the potential of insulin to stimulate glucose disposal. Blood glucose levels and plasma insulin levels were determined following an i.v. bolus of glucose (0.5g/kg; IVGTT) and the area under the curve for glucose (Aglu, in mg/dl*90 min), and for insulin (Ains mU/ml*90 min) were calculated. The Aglu did not differ between the first and second IVGTT following a bolus of saline (3504±821 (mean±SD) versus 3823±1074), nor the Ains (1248±437 versus 1424±377). In contrast, 60 minutes following a bolus of hyaluronidase (500 U) Ains increased significantly (1128±646 versus 1818±772 (p<0.05)), without a change in Aglu (3171±539 versus 3090±681). In conclusion, enzymatic degradation of the glycocalyx resulted in a 60% increase in plasma insulin required for normal glucose disposal. This finding is consistent with an important contribution of the glycocalyx to the ability of insulin to stimulate glucose uptake. Supported by DFN 2006.00.027 and NHF 2005T037