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Descending outer medullary vasa recta are constricted by perfusion with iodinated contrast media
Author(s) -
Sendeski Mauricio Michalak,
Patzak Andreas,
Pallone Thomas L.,
Cao Chunhua,
Persson Erik,
Persson Pontus B.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.949.1
Subject(s) - iodixanol , perfusion , vasa recta , chemistry , renal medulla , kidney , vasoconstriction , iopamidol , oxidative stress , nitric oxide , medullary cavity , contrast medium , medicine , radiology
Objective Contrast induced nephropathy (CIN) is a frequent complication of coronary angiography. Outer medullary descending vasa recta (DVR) are contractile microvessels supplying blood flow to the renal medulla ‐ the area of kidney at risk for CIN. Our objective was to test whether perfusion of DVR with a contrast medium (CM) modifies DVR vasoactivity. Methods DVR from rats were microscopically isolated and perfused with iodixanol (23 mg iodine/ml, calculated after usual doses of CM). DVR luminal diameter was determined by microscopy and nitric oxide (NO) was measured by fluorescent techniques. Results CM led to 50% reduction of luminal diameter, and increased vasoconstriction of DVR by angiotensin II (ANG II). Dismutation of superoxide by Tempol prevented these effects. CM also decreased NO bioavailability by >60 percent. Discussion Our data suggest that CM may lead to renal circulatory derangements described in CIN through a direct effect on DVR. This effect seems to happen via increased oxidative stress. Scavenging of reactive oxygen species might exert a therapeutic effect on CIN by mildering such derangements. Supported by the German Research Foundation, Werner Jackstaedt Foundation, and NIH