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In vitro system for studying blood, perfluorocarbons (PFC) and Sickle Cell Anemia
Author(s) -
Narayanan Srinivasan Vasudevan,
Berger Brian,
Torres Filho Ivo
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.948.21
Subject(s) - hemoglobin , chemistry , oxygenation , oxygenator , red blood cell , sickle cell anemia , blood volume , anesthesia , cell , medicine , biochemistry , cardiopulmonary bypass
PFCs are inert compounds that dissolve more oxygen (O 2 ) than plasma. A mixture of PFC and sickle cell blood could potentially hold and deliver more O 2 to tissues, compensating for sickle cell red blood cell's (RBC) decreased affinity for O 2 . A system was developed for in vitro studies using blood mixed with PFC. The system (priming volume: 10 ml) includes a computer‐controlled pump, gas mixer, oxygenator, deoxygenator and interconnected tubing that allowed the flowing blood to be exposed to various O 2 and CO 2 tensions, yielding controlled levels of hemoglobin O 2 saturation (SO 2 ) and O 2 partial pressures (PO 2 ). SO 2 and PO 2 were measured using a blood gas analyzer and a CO‐oximeter. With the addition of 5, 10, and 20% of PFC by volume of blood, the total O 2 content, O 2 delivery and consumption increased linearly with the PFC concentration. The data suggest increased O 2 transport by the PFC. The SO 2 of blood mixed with PFC did not differ significantly from baseline. Changes in blood cell morphology can also be evaluated at each oxygenation level and PFC concentration using video‐microscopy. The percentage of RBC that sickle at a given PO 2 with and without PFC will be determined. The system allows several variables to be studied using blood sampled from animals and patients. Support: Virginia Commonwealth University.