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Effect of quercetin on reactivity of sub‐chronic cadmium‐exposed rat aorta
Author(s) -
Trakranrungsie Nopamart,
Yatmark Paranee,
Akkhawattanangkul Yada
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.933.13
Subject(s) - quercetin , chemistry , cadmium , aorta , angiotensin ii , endocrinology , pharmacology , medicine , acetylcholine , vascular smooth muscle , bradykinin , antioxidant , biochemistry , smooth muscle , receptor , organic chemistry
The effect of quercetin, a potent radical scavenging flavonoid known to associate with reduced risk of cardiovascular disease, on reactivity of rat aorta was investigated in a sub‐chronic, orally cadmium‐exposed model. Isometric vascular measurement was employed to access vascular responses to vasoactive substances: acetylcholine (ACh), bradykinin (BK), angiotensin II (AT II) and endothelin‐1 (ET‐1). Cadmium (Cd) exposure, at 10, 30 and 100 ppm, via drinking water for 8 weeks did not cause an alteration in reactivity to ACh, but reduced relaxation response to BK in rats receiving 30 ppm Cd. Co‐treatment with 50 mg/kg quercetin could significantly recover vascular BK responses. Exposure to Cd appeared to have dual effect on aortic response to AT II. At 10 ppm Cd, contraction induced by AT II was partially impaired, while at higher dose (30 ppm), the response was potentiated, particularly at 10 −10 M AT II. Although aortic rings from rats receiving quercetin alone demonstrated the marked loss in AT II response, in Cd‐treated rats, quercetin could restore the overall vascular reactivity. In addition, reactivity of aorta to ET‐1 was notably reduced by Cd, of which the effect could be reversed by quercetin. Results also showed that exposure to Cd increased levels of serum GGT and Cd accumulation in kidneys and liver in a dose‐dependent manner. Quercetin treatment reduced Cd burden in kidney only at low dose of Cd administration.