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Bronchial oxidative stress in equine recurrent airway obstruction
Author(s) -
Venugopal Changaram,
Mariappan Nithya,
Holmes Earnestine,
Francis Joseph,
Eades Susan
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.931.4
Subject(s) - oxidative stress , reactive oxygen species , pentoxifylline , tumor necrosis factor alpha , inflammation , medicine , pathogenesis , chemistry , pharmacology , immunology , pathology , endocrinology , biochemistry
Oxidative stress is an important characteristic of hyperactive airway diseases such as recurrent airway obstruction (RAO) in horses. The primary source of oxidative stress in RAO is from the elevated amounts of reactive oxygen species (ROS) liberated from the inflammatory cells. ROS alters signal transduction mechanisms, activate redox‐sensitive transcription factors and regulate pro‐inflammatory gene expressions. Thus, ROS levels have important consequences for pathogenesis, severity and therapeutic management of RAO. The involvement of Tumor necrosis factor‐alpha (TNF alpha) and NF kB are well established in inflammation. Therefore, the objective of the study was to determine oxidative stress in pulmonary bronchial rings of RAO‐affected and unaffected horses and to test the effectiveness of TNF alpha blockade and NF KB to reduce production of ROS. To block TNF‐alpha and NF KB we incubated the rings with Pentoxifylline and PDTC (Pyrrolidinedithiocarbamate), respectively. Bronchial rings were collected from right diaphragmatic lung lobe of RAO‐affected and unaffected horses and the ROS released by the tissues were measured in control tissues and those incubated with the antagonists, using Electron Paramagnetic Resonance (EPR). The results showed that total ROS was greater in RAO‐affected than the unaffected horses. In addition, incubation of the rings with Pentoxifylline and PDTC reduced the ROS level in both groups of horse rings. The study is suggestive of therapeutic value for these agents in RAO‐affected horses to reduce oxidative stress. Supported by LSU‐SVM Equine Health Studies Program.