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Differential Effect of Leptin on Adipose Tissue‐Derived Stromal Cells and Bone Marrow‐Derived Stromal Cells
Author(s) -
jang hoon,
hong seong chul
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.930.8
Subject(s) - leptin , adipocyte , adipose tissue , stromal cell , mesenchymal stem cell , endocrinology , bone marrow , medicine , chemistry , microbiology and biotechnology , biology , obesity
The adipocyte actively gives effect to feedback of surroundings and storing excess energy in the form of triglycerides. These cells secrete leptin that regulate food intake because it acts on central nerve system. Also these cells are used by surgery and plastic surgery. The adipocyte has not ability of proliferation which is formed by differentiation from mesenchymal stem cells (MSCs). Recently the concern and increasing of plastic surgery need more adipocyte, but fat poverty patients were hard under the knife of transplant operation, because who have not surplus fat due to transplant. Therefore we tested differentiation effect of rat MSC by induced media which include various sources each of cholesterol, vegetable fat, animal fat and leptin. we confirmed that leptin stimulate to differentiate into adipocyte by GPDH assay. But several studies demonstrated that leptin inhibit the BMSCs to differentiate into adipocyte. Therefore we test dose response of concentration of leptin for differentiation into adipocyte. As a result, 500ng/ml leptin concentration has the most of effect on differentiation of ATSC and BMSC. These results suggest that ATSCs and BMSCs have ability of differentiation.