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Prostate cancer cells home to bone in a new in vivo model of bone metastasis
Author(s) -
Reeves Kimberley Jayne,
Pluijm Gabri,
Cecchini Marco G,
Eaton Colby L,
Hamdy Freddie C,
Brown Nicola J
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.927.11
Subject(s) - extravasation , metastasis , prostate cancer , angiogenesis , histology , bone metastasis , in vivo , medicine , pathology , anatomy , homing (biology) , cancer , chemistry , biology , cancer research , microbiology and biotechnology , ecology
This study aimed to establish the dorsal skinfold chamber (DSC) model of prostate cancer metastasis. Metatarsals from newborn mice are engrafted into a DSC implanted a SCID mouse (5‐6 weeks old). We have demonstrated that metatarsals are rapidly vascularised by inosculation with the host vasculature by day 5‐7, with functional vessels by day 2. Vascular density was measured over a 7 day period; day 3, 455 ± 186 cm/cm 2 ; day 5, 546 ± 305 cm/cm 2 and day 7, 707 ± 370 cm/cm 2 . Following this fluorescently labelled prostate (PC3‐GFP) or oral (SCC4‐GFP) cancer cells (1 x 10 5 ) are injected via the heart to simulate metastatic spread. Cells initially adhere to the microvascular endothelium and/or to metatarsal matrix 5 days after injection (10 ± 7 cells/metatarsal). Bone viability has been assessed throughout the 4 week study duration and demonstrates viable tissue within the DSC. Histology of the metatarsal confirms the presence of tumour cells, viability of the metatarsal, active growth plate and new vessels formation. This model will allow homing, interactions and extravasation between the tumour and bone microenvironment to be studied in detail. Funded by Sixth European Framework Programme (PROMET).