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The Levels of Calcitonin Gene‐Related Peptide and Substance P in the Plasma of Rats with Traumatic Brain Injury and the Role of Neurogenic Inflammation in the Pathogenesis of TBI
Author(s) -
Duan hubin,
Hao chunyan,
Li shuzhen
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.926.2
Subject(s) - calcitonin gene related peptide , traumatic brain injury , substance p , medicine , neurogenic inflammation , calcitonin , neuropeptide , pathogenesis , endocrinology , inflammation , receptor , psychiatry
Background and Purpose Substance P (SP) is thought to play a crucial role in the traumatic brain injury (TBI). Calcitonin gene‐related peptide (CGRP) is an inflammatory neuropeptide found in neurons. The purpose of research is to explore the correlation between SP and CGRP in the rats plasma after TBI and to study their significance in the neuropathic mechanism of TBI. Methods l68 male Wistar rats were randomized into the injury group and control group.Mild,moderate and severe injury models (0.5‐1.0 atm,1.5‐2.0 atm and 2.5‐3.0atm ) was produced with the fluid percussion brain injury (FPI) technique in anesthetized adult rats equipped with a closed cranial window.All groups were again stratified into 7 sub‐groups according to the time after TBI(0.5,2,6,12,24,48 and 72 h).The plasma levels of SP and CGRP were measured with ELISA at specific time after the injury. Results There was a positive correlation between the plasma levels of SP and CGRP (r=0.879.P<0.01)after TBI.The plasma levels of SP and CGRP varied significantly across all groups with different severity of TBI(P<0.01) and time after injury (P<0.01).The peak and trough of plasma levels of SP were recorded in 0.5 h‐moderate (1.46±0.23ng)and 24h‐severe (0.43±0.17ng) sub‐groups,and those of CGRP were recorded in 2 h‐moderate (5.9±0.9ng)and 24 h‐severe sub‐groups(0.7±0.4ng) respectively. Conclusion These data show that both plasma levels of SP and CGRP changed regularly with the severity of injury and the time after TBI. A positive correlation also be observed between the plasma levels of SP and CGRP.Therefore, detection of plasma SP and CGRP should be helpful to estimate the severity of injury and pathological changes of the TBI.