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Zinc (Zn) Modulates the Stress Response in Cadmium (Cd) Treated Cultured Choroid Plexus
Author(s) -
Bradley Jennifer L,
Young Robin K,
Villalobos Alice R
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.922.7
Subject(s) - phalloidin , dapi , cytoskeleton , hsp70 , actin , chemistry , choroid plexus , microbiology and biotechnology , biology , biophysics , cell , heat shock protein , biochemistry , endocrinology , apoptosis , gene , central nervous system
Our objective is to elucidate how an essential mineral Zn might attenuate the cellular stress response elicited by a contaminant metal, e.g., Cd, in choroid plexus (CP). To begin, primary cultures of neonatal rat CP were supplemented ± 25 µM Zn with 5% NuSerum for 48 h then exposed to 500 nM Cd ± 10 µM Zn in serum‐free medium for 24 h. As per immunoblot analysis, Hsp70 and hemeoxygenase‐1 (HO) were expressed at low levels in control cells (no Zn/no Cd) and cells treated only with Zn. Cd increased Hsp70 and HO by 3‐6‐fold, but co‐exposure with Zn attenuated induction of both stress proteins. Hsp70 expression was also imaged by immunochemistry and fluorescence microscopy; DAPI was used for nuclear staining. Diffuse low fluorescence was detected in controls and in cells treated only Zn. Cd increased Hsp70 throughout the cytosol and altered cell morphology, but in cells co‐exposed to Zn Hsp70 expression pattern was similar to that in controls. Actin cytoskeleton was probed with TRITC‐phalloidin. In control cells, actin filaments were localized at the periphery, whereas in cells treated with Cd plus Zn there was an increase in actin filaments throughout the cell. Interestingly, in cells treated only with Zn actin filaments were localized at the periphery as in control. These preliminary data suggest that Zn attenuates induction of stress proteins and modifies changes in the actin cytoskeleton in Cd‐treated CP.