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Characterizing the chelatable/labile iron pool in mammalian cells
Author(s) -
Hill Natasha,
Rojas Ben,
Thon Vireak,
Beck Albert,
Tonnu Lena,
Planutyte Lina,
Mason Andrew Z.,
Linder Maria C.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.921.10
Subject(s) - chemistry , ferritin , biophysics , cytosol , peptide , fragmentation (computing) , cell , tracer , biochemistry , enzyme , biology , ecology , physics , nuclear physics
Iron shuttling in and out of cellular proteins and compartments comprises a chelatable or labile pool. Attempts at characterizing this pool have been largely unsuccessful. Using 3h labeling with tracer 59 Fe, we identified a 1600 Da component in ultrafiltrates of cultured cell lysates that degraded to 600 Da over time (if not separated from the bulk of the cellular protein) and could be converted from 1600 to 600 Da with proteinase K. Both the 1600 and 600 Da components were negatively charged at pH 7+ and separated from cellular 59 Fe‐labeled ferritin (Ft) in native PAGE. 59 Fe in the unknown versus Ft varied inversely with total cellular Fe. However, analysis of Centricon 10 ultrafiltrates from human HepG2 and Caco2 cells showed that the Fe content did not vary appreciably with cellular Fe status (pretreatment with 180 uM Fe or 30 uM DFO for 24h, or untreated). Concentrations in this pool were 90‐ 200 ng/mg cell protein, with total iron contents ranging from 200 to1600 ng/mg. Mass spectrometry after laser fragmentation released multiple units of 44 Da (consistent with COO‐). We conclude that the cellular labile Fe pool stays quite constant despite changes in Fe status and may primarily consist of a complex of Fe with a small peptide plus carboxylate units. Supported in part by PHS Grant No. RO1 HD 46949.