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Intragastric infusion of arginine stimulated greater liver protein synthesis compared to IV infusion in TPN‐fed Yucatan miniature piglets
Author(s) -
Dinesh Chandani,
Dodge M Elaine,
Baldwin Mark P,
Bertolo Robert F,
Brunton Janet A
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.919.4
Subject(s) - arginine , medicine , endocrinology , parenteral nutrition , protein biosynthesis , biology , chemistry , biochemistry , amino acid
Total parenteral nutrition (TPN) induced gut atrophy reduces de novo synthesis of intestinal arginine (Arg) in neonates which may limit Arg availability. Our objective was to assess the effect of route of Arg intake on tissue protein synthesis in TPN‐fed piglets with gut atrophy. Piglets (14‐17 d) were fed TPN (1.0 g Arg/kg/d) until study d 4, then switched to Arg‐free TPN and randomized to receive a continuous intragastric infusion (2 mL/kg/hr) of either IG Low Arg (0.6 g/kg/d) or IG High Arg (1.6 g/kg/d). A 3 rd group was switched to high Arg TPN (1.6 g/kg/d) (IV High Arg). A group of sow‐fed (SF) littermates was also included. On d 7, piglets received an IV flooding dose of 3 H‐phenylalanine to measure protein synthesis (Ks, %/d). Liver Ks was greater in IG High Arg compared to IV High Arg. Mucosal Ks was highest in SF animals, with no differences in the TPN‐fed groups. Table values are mean (SD), N=3‐6 per tissue and group. Different superscripts by row, p<0.05 (ANOVA) Provision of high dietary Arg alone into the gut did not appear to enhance mucosal protein synthesis. Muscle protein synthesis was also not different, but was highly variable. IG delivery of Arg promoted liver protein synthesis compared to IV feeding; the mechanism is likely not related to Arg availability as there was no difference in liver free Arg. (CIHR)