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Arginine supplementation improves insulin resistance in obese adolescents
Author(s) -
Verbruggen Sascha,
CossBu Jorge,
Hsu Jean WCh,
Joosten Konrad,
Goudoever Johanness,
Castillo Leticia
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.914.7
Subject(s) - medicine , insulin resistance , arginine , endocrinology , insulin , lipolysis , glucose clamp technique , pancreatic hormone , chemistry , amino acid , adipose tissue , biochemistry
Arginine through a NO mediated mechanism improves insulin resistance in type 2 diabetes. To asses the effect of a short‐term (1 week) dietary arginine supplementation on insulin resistance in glucose intolerant obese adolescents, we conducted a randomized, cross‐over study in 12 subjects (16±1 yr.;BMI 36±5 kg/m 2 ) receiving a one week diet period of a weight maintaining protein and energy intake, or an isocaloric arginine supplemented (21 gram/day) isonitrogenous diet. At the end of each diet period they received a primed, constant, 7 hr. tracer infusion of [1‐ 13 C]Glucose, L‐[ 2 H 3 ]Leucine and [ 2 H 5 ]Glycerol. During the last 3 hours a hyperinsulinemic euglycemic clamp (HEC; 40 mU·m −2 ·min −1 insulin) was conducted. Arginine supplementation improved (p<0.05) insulin sensitivity from 0.3±0.2 to. 0.4±0.2 μmol ·kg −1 ·min −1 /μU/mL. There was a trend towards improvement of non‐oxidative glucose disposal (3.5±3 vs. 4.5±2 mg.kg −1 .min −1 ; p=0.06). However, there was no effect on endogenous glucose production (‐1.0±1 vs. ‐1.0±2 mg.kg −1 .min −1 ; p=0.99). Arginine had no effect on protein breakdown or lipolysis. A one week oral arginine supplementation (21 gr/day) in obese adolescents improves insulin resistance. NIH DK‐62363, T32 HD‐007445, Ajinomoto 3‐ARP; USDA/ARS 25337387; Sophia Foundation for Scientific Research (SSWO).