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n‐3 Polyunsaturated Fatty Acids Suppress T‐Cell Mitochondrial Translocation to the Immunological Synapse
Author(s) -
Yog Rajeshwari,
Barhoumi Rola,
McMurray David N.,
Chapkin Robert S.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.910.20
Subject(s) - immunological synapse , t cell , microbiology and biotechnology , mitochondrion , polyunsaturated fatty acid , cytoplasm , biology , cell , immune system , chemistry , t cell receptor , biochemistry , immunology , fatty acid
T helper (T h ) cell activation is necessary for the adaptive immune response. Formation of the immunological synapse (IS) between T h cells and antigen‐presenting cells is the first step in T h cell activation. In vitro studies indicate that formation of the IS induces cytoskeleton‐dependent mitochondrial redistribution to the immediate vicinity of the IS. This redistribution of mitochondria to the IS in T cells is necessary to maintain Ca 2+ influx across the plasma membrane and Ca 2+ ‐dependent T h cell activation. In an earlier study, we demonstrated that n‐3 polyunsaturated fatty acids (PUFA) suppress the localization and activation of signaling proteins at the IS. Therefore, we hypothesized that n‐3 PUFA suppress CD4 + T‐cell mitochondrial translocation during the early stages of IS formation. CD4 + cells derived from fat ‐1 mice, a transgenic model that synthesizes n‐3 PUFA from n‐6 PUFA, were co‐cultured with anti‐CD3‐expressing hybridoma cells (145‐2C11) for 15 min at 37°C, and mitochondrial relocation to the IS was assessed by confocal microscopy. T‐cells from fat‐1 mice significantly reduced the percentage of cells with mitochondria which translocated to the IS; fat ‐1 (34%) vs. wild type control (85%); P=0.008. These results demonstrate that n‐3 PUFA limit mitochondrial translocation to the IS, a critical early step for CD4 + T‐cell activation. Supported in part by NIH grant DK07107, CA59034 and P30ES09106 Grant Funding Source NIH DK71707 and CA59034