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Tea catechins reduce viability and cytokine‐induced inflammation in gastric cancer (AGS) cells
Author(s) -
Gutierrez Orozco Fabiola,
Green Rodney J,
Ferruzzi Mario,
Bomser Joshua A
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.910.10
Subject(s) - viability assay , intracellular , catechin , inflammation , cytokine , pharmacology , chemistry , secretion , cancer , cancer cell , apoptosis , cancer research , medicine , microbiology and biotechnology , immunology , biochemistry , biology , antioxidant , polyphenol
Chronic inflammation is involved in the development of gastric cancer, the second leading cause of cancer‐related death worldwide. Epidemiological evidence suggests that increased consumption of catechin rich tea may be associated with reduced risk of gastrointestinal cancers. The goal of this study was to evaluate the effects of catechin‐rich extracts of green (GT) and black tea (BT) and individual tea catechins (EGC, EGCG, EGC/EGCG) on cell viability, intracellular oxidation and inflammation in the AGS gastric cancer cell line. Cell viability was measured using the MTT assay. Intracellular oxidation was evaluated using the probe dichlorofluorescin. Inflammatory markers included measurement of intracellular and secreted IL‐8 protein and the activation of nuclear factor‐kappa B (NFκB). Treatment of AGS cells (48h) with EGC, EGC/EGCG and EGCG, reduced cell viability by 36%, 31% and 19%, respectively. Interestingly, a similar reduction in cell viability (27%) was observed upon treatment of cells with BT and GT extracts. Treatment of cells with GT (1.5 mg/ml), BT (1.5 mg/ml) and EGCG (1.5 mg/ml) also inhibited cytokine‐induced IL‐8 production and subsequent secretion. These anti‐inflammatory effects are due, in part, to a reduced activation of NFkB in the AGS cell line. This study demonstrates a potential mechanism by which tea catechins reduce gastric inflammation.