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Conjugated linoleic acid reduces joint inflammation in a murine collagen‐induced arthritis model
Author(s) -
Huebner Shane M,
Campbell James,
Loy Meaghan,
Butz Daniel E,
Cook Mark E
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.909.4
Subject(s) - conjugated linoleic acid , arthritis , inflammation , chemistry , linoleic acid , rheumatoid arthritis , collagen induced arthritis , immune system , medicine , corn oil , endocrinology , immunology , biochemistry , food science , fatty acid
Previously, mice fed mixed isomers of conjugated linoleic acid (CLA) had reduced antibody‐dependent inflammation in two murine models of arthritis. The objective of this study was to determine the CLA isomer responsible for reduced inflammation in the collagen‐induced model of arthritis (CIA). To avoid CLA's effects on the acquired immune response, arthritis was first induced in mice by a chick type II collagen injection. Post inflammation, mice were randomized to one of four diets: 1.0% corn oil (control); 0.5% c9,t11‐CLA; 0.5% t10,c12‐CLA; or 1.0% mix‐CLA isomers. The severity of arthritis was determined both subjectively (arthritic score) and objectively (paw thickness). The absolute increase in paw thickness due to arthritis at 12 weeks (sham injected/control fed versus collagen‐injected/control fed) was 0.22mm. At 12 weeks, the reduction in paw swelling due to CLA feeding was 33.4, 66.3, and 39.5% for mice fed c9, t11‐CLA, t10,c 12‐CLA, and mixed isomers, respectively. The reduction in arthritic scores, relative to control fed/collagen‐injected mice, due to CLA feeding was 39.7, 57.1, and 37.5% for mice fed c9,t11‐CLA, t10,c12‐CLA, and mixed isomers, respectively. Hence, both isomers of CLA had anti‐inflammatory properties in this murine CIA model. The t10,c12‐CLA isomer was more effective than either the t9,c12‐ or mixed‐CLA isomers as an anti‐inflammatory treatment. Research was supported by a Hatch Grant.