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Splenic dendritic cell populations in vitamin A deficient C57BL/6J mice
Author(s) -
Duriancik David Michael,
Hoag Kathleen Ann
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.909.2
Subject(s) - myeloid , immune system , dendritic cell , vitamin , biology , immunology , flow cytometry , immunity , t cell , acquired immune system , spleen , vitamin d and neurology , endocrinology
Vitamin A‐deficient individuals have altered adaptive immunity, showing depressed antibody‐mediated [T helper (Th)2] responses and enhanced cell‐mediated (Th1) responses. In the mouse, myeloid dendritic cells (DCs) and lymphoid DCs are responsible for stimulating Th2 and Th1 responses, respectively. The objective of this research project was to compare the percentage and absolute numbers of DC populations in vitamin A‐sufficient and vitamin A‐deficient uninfected laboratory C57BL/6J mice. The hypothesis was that vitamin A‐deficient animals would have decreased myeloid DCs and increased neutrophils and precursor DCs compared to vitamin A‐sufficient animals. We used dietary restriction to deplete the animals of vitamin A and multicolor flow cytometry to detect and quantify the numbers of immune cell populations from individual mouse spleens. In accordance with our hypothesis, the myeloid DCs are decreased and the neutrophils and precursor DCs are increased in the vitamin A‐deficient animals compared to the vitamin A‐sufficient animals. Therefore, the observed decrease in myeloid DCs in vitamin A‐deficient animals may be the basis for depressed Th2 function in vitamin A‐deficient animals. Grant Funding Source Department of Food Science and Human Nutrition, Michigan State University