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Effect of diets high in fats or carbohydrates on the immune system of young BALB/c mice
Author(s) -
MartínezCarrillo Beatriz E,
ZúñigaTorres Guadalupe M.,
ValdesRamos Roxana,
RiveraAguilar Victor,
JarilloLuna Rosana A,
CamposRodríguez Rafael
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.907.8
Subject(s) - cd8 , immune system , chemistry , analysis of variance , cd3 , lamina propria , carbohydrate , medicine , endocrinology , biology , biochemistry , immunology , epithelium , genetics
Increased dietary fat or carbohydrates affects metabolism, the immune system is no exception. Objective To evaluate the effect of dietary increase in fat or carbohydrate content on the immune system of a mouse model. Methods 24 Balb/c mice were divided in three groups at 21 days of age. Control (C), high‐carbohydrate (HCO) or high fat (F) diets were fed ad libitum for 9 weeks. Intra‐ocular blood and total small intestines were obtained for analyses of lymphocytes using anti‐CD3+, CD4+ y CD8+ antibodies by flow‐cytometry. Results CD3+ cells were higher in the HCO (18.10% ±1.51) and F groups (17.31% ±1.53), than the C group (12.40% ± 1.42) (ANOVA=34.15 and <0.001). No differences were found in CD4+ cells between any of the groups. CD8+ cells were higher in the F group (14.83% ±1.42) compared with the C group (11.06% ±1.32), but not with the HCO group (12.42 ± 0.9) (ANOVA=258.23 and p< 0.001). Lamina propria CD3+ cells were lower in the F group (30.92% ± 3.62) when compared with HCO‐CD3+ (56.36 ± 6.76) and C‐CD3+ (44.34 ± 3.84) (ANOVA=83.38, p<0.001). F‐CD4+ (1.48% ±0.333) and F‐CD8+ (3.46% ±1.87) were lower the C‐CD4+ (19.98 ± 3.59), C‐CD8+ (22.29 ± 3.82), HCO‐CD4+ (20.24 ± 4.5) and HCO‐CD8+ (26.93 ± 4.6) (CD4+ ANOVA=52.78, p<0.001; CD8+ ANOVA=92.71, p<0.001). Conclusions T‐lymphocytes are decreased in peripheral blood by high carbohydrate or low fat diets, and decreased in the small intestine by a diet high in fat.