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Effects of Moderate Zinc Deficiency and Phytase Supplementation on Lumbar Vertebrae in Exercising Rats
Author(s) -
Catrambone Daniel E,
Antczak Amanda J,
Hendrickson Nathan R,
Regalia Ryan S,
Young Andrew J,
Scrimgeour Angus G
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.906.4
Subject(s) - micronutrient , lumbar vertebrae , zinc , bone mineral , phytase , chemistry , medicine , zoology , bioavailability , quantitative computed tomography , endocrinology , lumbar , osteoporosis , anatomy , biochemistry , biology , enzyme , organic chemistry , pharmacology
Dietary zinc (Zn) is important for bone remodeling. Phytase (P) increases the bioavailability of Zn and other micronutrients. How modeling adaptations of the lumbar spine may be affected by a P supplemented low Zn diet has not been investigated. PURPOSE Determine the effects of a low Zn diet and supplemental P on the 4th lumbar vertebra (L‐4) in exercising rats. METHODS 30 Male Sprague‐Dawley rats were housed in cages fitted with voluntary running wheels and fed either a 5ppm Zn (ZnLo), 5ppm Zn + 1,500 FTU/kg P (ZnLo+P) or a 30ppm Zn (ZnAd) diet for 9 wks, (n=10 per group). In vivo peripheral quantitative computed tomography scans were performed at 40, 50 and 60% of the total L‐4 length. Cortical content (CNT) (mg), area (A) (mm 2 ) and bone mineral density (BMD) (mg/cm 3 ) were measured. Plasma concentrations (PC) (µg/ml) of Zn were determined. A one‐way ANOVA was used to compare the data. RESULTS ZnLo+P exhibited increased CNT (11.6 ± 1.5 vs. 9.9 ± 0.7, p=0.006), A (12.0 ± 1.4 vs. 10.5 ± 0.7, p=0.007) and BMD (959.5 ± 15.9 vs. 938.8 ± 7.9 p=0.012) compared to ZnLo and increased BMD (959.5 ± 15.9 vs. 942.5 ± 18.9, p=0.043) compared to ZnAd. ZnAd exhibited increased PC of Zn (1.2 ± 0.2 vs. 0.8 ± 0.1 and 0.5 ± 0.2, p<0.001) compared to ZnLo+P and ZnLo respectively. ZnLo+P exhibited increased PC of Zn (0.8 ± 0.1 vs. 0.5 ± 0.2, p=0.01) compared to ZnLo. CONCLUSION P supplementation increased PC of Zn and increased mid‐vertebral cortical CNT, A and BMD in a ZnLo fed sample. The greatest L‐4 cortical modeling was observed in rats fed a ZnLo+P diet. The study was funded by the U.S. Army MRMC