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Effect of high saturated‐fat diets on bone mass and endochondral ossification in healthy growing rats
Author(s) -
Macri Vanesa,
Juiz Natalia,
Gonzales Chaves Macarena,
Ponce Graciela,
Lanata Eduardo,
Rodríguez Patricia,
Mandalunis Patricia,
Zeni Susana,
Friedman Silvia
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.906.2
Subject(s) - endochondral ossification , medicine , endocrinology , weaning , bone mineral , zoology , chemistry , cartilage , biology , anatomy , osteoporosis
High‐fat diets can produce deleterious effects on bone metabolism in growing animals. Objective: To investigate in healthy growing rats the effect of high saturated‐fat diets on bone mass and endochondral ossification. Weaning male Wistar rats (n=20) were fed ad libitum one of the 2 diets, for 4 wks: control (C; fat=7% w/w) (AIN‐93G) or a high‐fat diet containing 20% w/w of beef‐tallow (BT). Then rats were sacrificed. Total skeleton bone mineral density (BMD) and bone mineral content (BMC) were evaluated by DXA (Lunar DPX‐L).The following histomorphometric parameters were evaluated: Bone volume (BV/TV)(%) and cartilage growth plate thickness (CGP.Th).Statistics: Student's t test & Pearson's correlations coefficients were calculated. Results (as mean±SD): BMD(mg/cm 2 ), C=245±13 vs BT=244±13 (p>0.05); BMC(mg), C=1085±319 vs BT=689± 224; (BV/TV)(%), C=45±5 vs BT=38±3 (p<0.03); total GPC.Th(mm), C=0.44±0.04 vs BT=0.43±0.03 (p>0.05); proliferative zone GPC.Th(mm), C=0.21±0.01 vs BT=0.18± 0.01 (p>0.001).The correlation between BMC vs proliferative zone CGP.Th was ‐0.881 (p<0.05). Conclusion High saturated‐fat diets lead to a decreased in BMC associated with a significant increased in CGP.Th as well as a reduction in BV/TV. Based on these findings, we suggest that high saturated‐fat diets acts as a dependent risk factor for bone modeling interfering the acquired of bone mass. Awarded by UBACyT O 008 ‐ O015.