Premium
The use of pyrosequencing to identify diet‐induced changes in the canine gut microbiome
Author(s) -
Middelbos Ingmar S,
Swanson Kelly S,
Qu Ani,
White Bryan A,
Fahey George C
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.905.4
Subject(s) - fusobacteria , firmicutes , pyrosequencing , biology , bacteroidetes , microbiome , unifrac , phylum , ruminococcus , proteobacteria , zoology , genetics , 16s ribosomal rna , gene
The influence of the gut microbiome on host health is becoming increasingly recognized. Although our knowledge of mammalian gut microbiomes is limited, new molecular techniques provide great opportunities. We used high throughput DNA 454‐pyrosequencing of amplicons from the hypervariable V3 region of the 16S rRNA gene to characterize gut microbiome composition in dogs fed two different diets. Six dogs were fed diets containing 0% (control; C) or 7.5% beet pulp (BP) in a crossover design with 14‐d periods. Estimates of individual microbiome diversity ranged from 198 to 513 species (cutoff: 96% similarity), slightly higher than that of humans. Three dominant bacterial phyla were detected, including Fusobacteria, Firmicutes, and Bacteroidetes. Fusobacteria decreased (P < 0.05) whereas Firmicutes increased (P < 0.05) when dogs were fed BP. UniFrac principal component analysis showed that microbiomes clustered by diet and that compositional changes in the phylum Firmicutes appeared to be a major contributing factor. These data suggest co‐dominance of three bacterial phyla in the dog gut compared to two in most mammals and that diet significantly altered gut microbiome composition.