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Effect of leucine feeding on body weight and carbohydrate metabolism in dietary fat‐induced obese rats
Author(s) -
Park Ji Min,
Park Hoon Jung,
Kim Mi Kyung
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.903.5
Subject(s) - medicine , endocrinology , carbohydrate , obesity , adipose tissue , weight loss , metabolism , leucine , body weight , chemistry , carbohydrate metabolism , biochemistry , amino acid
The aim of this study was to examine the effect of leucine on body weight and carbohydrate metabolism in 6 month‐old Sprague‐Dawley (n=80) rats. One group of 10 rats was fed control diet (control group) and another group of 70 rats was fed a high‐fat diet for 14 wks (high‐fat group) to induce obesity. Ten rats of each group were killed at this stage and the remaining obese rats were fed with one of six experimental diets containing 4%, 20% (w/w), two dietary fat levels (C‐, H‐) and three different Leu levels ; 0g, 0.6g, 1.2g /kg BW (‐LO, ‐LL, ‐LH) for 5 wks. Food intake, body weight, adipose tissues and organ weights were measured. To examine carbohydrate metabolism, oral glucose tolerance test (OGTT) conducted and the area under the curve (AUC) of each group was calculated. Body weight were significantly reduced in 1.2g/kg Leu groups (CLH, HLH) than 0g/kg Leu groups (CLO, HLO). Perirenal fat pad weights of 0g/kg Leu groups (CLO, HLO) were significantly higher than those of 0.6g/kg Leu groups (CLL, HLL) and those of 1.2g/kg Leu groups (CLH, HLH). AUC of 1.2g/kg Leu groups (CLH, HLH) were significantly lower than 0g/kg Leu groups (CLO, HLO). In conclusion, Leu administration might have weight loss effect and improve carbohydrate metabolism in dietary fat‐induced obese rats. * This work was supported by Sungshin Cement Co., Ltd. of Seoul, Korea and the second stage of the Brain Korea 21 project in 2008.