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The natural plant water extracts suppressed melanin production in murine
Author(s) -
You Jin Kyoun,
Seo Dong Joo,
Kim Dae Jung,
Chung Mi Ja,
Park Jeong Hae,
Choe Myeon
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.902.9
Subject(s) - tyrosinase , melanin , chemistry , antioxidant , dpph , traditional medicine , paeonia suffruticosa , skin whitening , camellia sinensis , biochemistry , food science , botany , pharmacology , enzyme , biology , active ingredient , medicine
Melanin is the pigment responsible for the color of human skin. Tyrosinase is known to be a key enzyme that catalyzes the synthesis of melanin in melanocytes. The effects of natural plant water extracts [( Camellia sinensis (BIC) , Black,allium sativum. L (HMN) , Aloe (ALE) , Crataegus pinnatifida (SS) , Paeonia suffruticosa (MDP) , Trichosanthes kirilowii Maxim (CHB) , Cornus officinalis (SSY) , Discorea japonica Thunb (SY ) ) on antioxidant, the activity of mushroom tyrosinase and melanin synthesis have been studied in a cell‐free system and mouse melanoma B16 cell. Radical‐scavenging activity was tested as reactivity towards 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH). The results showed that all the extracts exhibited antioxidant activity and the MDP, BIC and SSY extracts possessed high antioxidant among eight water extracts. The cellular tyrosinase activity and melanin content measured in mouse melanoma B16 cell by RT‐PCR and Western blot analysis. Treatment with HMN and ALE extracts significantly inhibited melanogenesis by tyrosinase. The melanin content of melanoma B16 cell was significantly decreased by treatment with MDP, BIC, HMN and ALE water extracts. Our results indicate that MDP, BIC, HMN and ALE water extracts exhibited antioxidant activity, tyrosinase and melanin reducing activities. The natural extracts may be a candidate for cosmetic use.