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Real‐time dynamics of pre‐mRNA transcription and splicing
Author(s) -
CarmoFonseca Maria
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.90.2
Subject(s) - spliceosome , rna splicing , minor spliceosome , intron , microbiology and biotechnology , polypyrimidine tract , splicing factor , transcription (linguistics) , exonic splicing enhancer , messenger rna , precursor mrna , rna , biology , genetics , gene , linguistics , philosophy
The spliceosome is a dynamic RNA‐protein macromolecular machine that is responsible for the splicing of intronic sequences from pre‐mRNA. The spliceosome undergoes major structural changes during the splicing reaction and its components must be recycled for each new round of splicing. Although the spliceosome cycle has been extensively studied at the molecular level, little is known about the dynamics of splicing and spliceosome assembly in living cells. We have been using Photobleaching and Photoactivation microscopy techniques to study how splicing factors are recruited to nascent transcripts in the living cell nucleus. Currently, we are developing tools to visualize in real‐time the dynamics of RNA Pol II transcription, splicing and spliceosome assembly.