Premium
Inhibitory effects of Aralia elata extract fractions on the biosynthesis of inflammatory mediators
Author(s) -
Kim GunHee,
Jeong ChoonSik,
Lee JeHyuk
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.899.2
Subject(s) - ethyl acetate , chemistry , antioxidant , traditional medicine , dpph , biochemistry , pharmacology , biology , medicine
Aralia elata ( A. elata ) has been used as a folk medicine for rheumatoid arthritis, diabetes, spasm of the stomach in Japan and Korea. Ethyl acetate‐fraction showed a scavenging activity of most DPPH free radical and reducing power of extract‐fractions showed the similar results. All A. elata extract fractions inhibited LPS‐induced nitrite production in a dose‐dependent manner. In the cell‐free system, all extract fractions chemically quenched NO partially, except aqueous fraction. Also, the iNOS mRNA transcription was suppressed in LPS‐stimulated RAW 264.7 cells. Especially ethyl acetate fraction (100 ¥ μg/mL) suppressed iNOS mRNA transcription to the basal level. In addition, all A. elata extract fractions showed the inhibitory effect on the production of prostaglandin E 2 and suppressed the corresponding COX‐2 mRNA transcription, except chloroform fraction. The production of interleukin‐1¥â and ‐6 were inhibited by methanol extract, ethyl acetate fraction, and butanol fraction to almost basal level. Based on these results, A. elata methanol extract fractions may be useful as an alternative medicine for the relief and retardation of immunological inflammatory responses through the reduction of inflammatory mediators, including NO, PGE 2 , and cytokines.