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Inhibition of fatty acid synthase by peptides from hydrolysates of β‐conglycinin enriched soybean
Author(s) -
Roman Maxine,
MartinezVillaluenga Cristina,
Bringe Neal A,
Gonzalez de Mejia Elvira
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.897.4
Subject(s) - fatty acid synthase , hydrolysate , enzyme , apoptosis , fatty acid , fas receptor , gene expression , messenger rna , chemistry , biochemistry , fatty acid binding protein , in vitro , fatty acid synthesis , gene , endogeny , microbiology and biotechnology , biology , programmed cell death , hydrolysis
The synthesis of fatty acids is a target pathway for chemotherapy development because it has been linked to the growth and survival of cancer cells. The inhibition of fatty acid synthase (FAS) causes apoptosis of cancerous cells. Due to the chemical instability and negative side effects of existing FAS inhibitors, it is important to search for alternative inhibitory sources. The objectives of this study were to study the inhibitory effect of soybean hydrolysates (SH) derived from β‐conglycinin (BC) enriched soybean on FAS and characterize the responsible peptides. Total RNA from 3T3‐L1 adipocytes was isolated and FAS RNA expression was measured using a real‐time quantitative PCR method with SYBR green fluorescence dye. SH (100 μM) derived from soybean enriched BC (up to 47% total protein) showed a marked decrease of FAS mRNA abundance relative to the negative control. However, SH from 24% BC did not affect FAS gene expression (P < 0.0001, R 2 = ‐0.90). It was concluded that soybean enriched in β‐conglycinin generates peptides that down regulate gene expression of enzymes involved in fatty acid synthesis in vitro , which can inhibit FAS. The potential health implications of this observation warrant further investigation. Supported by The Monsanto Company, Cargill Co. and University of Illinois