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Effects of dietary supplementations of tomato extract vs. lycopene on nonalcoholic steatohepatitis related hepatocarcinogenesis in rats
Author(s) -
Wang Yan,
Ausman Lynne M,
Greenberg Andrew S,
Russell Robert M,
Wang Xiangdong
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.897.23
Subject(s) - nonalcoholic steatohepatitis , medicine , chemistry , endocrinology , proinflammatory cytokine , liver cancer , lycopene , lipid peroxidation , inflammation , biochemistry , fatty liver , cancer , nonalcoholic fatty liver disease , antioxidant , oxidative stress , disease
Higher intake of tomatoes enriched in lycopene (LY) has been associated with lower risk for a variety of cancers. We recently showed that high‐fat diet (HFD) induced nonalcoholic steatohepatitis (NASH) and promoted diethylnitrosamine (DEN)‐initiated hepatocarcinogenesis in a rat model (Wang Y et al., Int. J. Cancer 2009). Using the same model, we investigated the efficacy of equivalent dosage of dietary LY from either tomato extract (TE) or purified LY against NASH related early hepatocarcinogenesis. Six groups of rats (n=8/group) were injected with DEN and then fed either Lieber‐DeCarli control diet or HFD with or without TE or LY for 6 weeks. Results showed similar plasma and hepatic LY concentrations between TE and LY groups, moreover, both TE and LY supplementations in the HFD groups significantly reduced altered hepatic foci multiplicity, lipid peroxidation, expressions of PCNA and cyclinD1, phosphorylated‐ERK, and nuclear NF‐kB p65 subunit in the liver, as compared with the HFD alone. However, TE, but not LY, inhibited HFD‐upregulated inflammatory foci and mRNA levels of proinflammatory cytokines (TNFalpha, IL‐1beta, IL‐12, CD14) in the liver. These data indicate that both TE and LY inhibit NASH related early hepatocarcinogenesis whereas TE provides an additional anti‐inflammatory potency due to a possible synergistic effect of multiple constituents in tomatoes (Supported by NIH grant R01CA104932). Grant Funding Source NIH grant R01CA104932