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Effects of isoangustone A isolated from hexane/ethanol extract of Glycyrrhiza uralensis (HEGU) on cell cycle progression in DU145 human prostate cancer cells
Author(s) -
Seon MiRa,
Hong Ji Eun,
Lim Soon Sung,
Kim JongSang,
Park Jung Han Yoon
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.897.20
Subject(s) - du145 , glycyrrhiza uralensis , glycyrrhizin , chemistry , pharmacology , cell cycle , cell growth , cancer cell , apoptosis , biochemistry , cancer , medicine , lncap , alternative medicine , pathology
Glycyrrhiza uralensis (licorice) has been shown to exhibit various pharmacological effects including antivirus, anti‐allergic and anti‐tumor effects. However, chronic consumption of high amounts of glycyrrhizin in licorice is known to induce undesirable hypermineralocorticoid syndromes, such as hypertension and hypokalemia. We previously prepared a hexane/ethanol extract of Glycyrrhiza uralensis (HEGU) which lacks glycyrrhizin and observed that HEGU induced apoptosis and cell cycle arrest in DU145 human prostate cancer cell growth. This study examined the effects of the active compound isoangustone A in HEGU on cell cycle progression of DU145 cells. Isoangustone A was obtained by subjecting the HEGU to a silica gel column and eluting by n‐hexane‐ethyl acetate gradient systems. Isoangustone A (2.5 ‐ 7.5 mg/L) dose‐dependently decreased the viable cell numbers and induced a G1 phase arrest. Isoangustone A reduced the levels of cyclin‐dependent kinase (CDK) 2, CDK 4, cyclin A, and cyclin D1 proteins. However, isoangustone A did not affect the levels of the cyclin E protein. These results indicate that isoangustone A induces G1 phase arrest in DU145 human prostate cancer cells by inhibiting CDK activities.