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Effects of curcumin on Lung cancer; Metabolomic Approach
Author(s) -
Goja Arvind,
Zaheeruddin Shaistha,
Mateescu Andreea,
Sadaat Nadia,
Gupta Smiti V.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.897.15
Subject(s) - curcumin , metabolite , metabolomics , lung cancer , carcinogen , cancer , chemistry , phytochemical , medicine , pharmacology , biochemistry , chromatography
Prognosis of lung cancer, the leading cause of cancer mortality in the US improves if detected at an early stage. Curcumin, a dietary phytochemical has proven anti‐inflammatory, antioxidant, and anti‐carcinogenic activity in several cancer types. This study aims to evaluate the effect of dietary curcumin in lung cancer induced hamster model, using a metabolomics approach. 32 male Golden Syrian hamsters were randomized into treatment (n=20; cancer induced by injecting N ‐nitrosobis‐2‐(oxopropyl) amine (BOP);12 weeks), and control (n=12) groups. Each group was subdivided into no (T0, C0) or 2% curcumin (T2, C2) diet groups. Proton nuclear magnetic resonance spectroscopy was conducted on 24h urine samples, in Deuterium oxide using a 700 MHz instrument at 25ºC. The NMR spectra were processed and analyzed using Principal Component Analysis (PCA; SIMCA P+, Umetrics), an unsupervised multivariate analysis technique. The PCA score plots showed clear separation between spectra from(i) the treatment (T0) and control (C0) groups (ii) groups with (T2) and without (T0) dietary curcumin (p < 0.05). The metabolites responsible for the inter‐group differences were quantified using the CHENOMX metabolite database. These metabolites, in addition to providing a mechanistic insight into cancer progression and/or inhibition, may serve as biomarkers for early diagnosis, progression and treatment of lung cancer.