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Synergistic effects of Curcumin and Garcinol on human pancreatic cancer cells
Author(s) -
Parasramka Mansi A.,
Wrubel Jennifer Ann,
Gupta Smiti V.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.897.14
Subject(s) - curcumin , dapi , apoptosis , pancreatic cancer , trypan blue , chemistry , cancer cell , mtt assay , cancer , cancer research , pharmacology , cell cycle , viability assay , flow cytometry , biology , biochemistry , microbiology and biotechnology , medicine
Pancreatic cancer (PaCa) is the fourth leading cause of cancer related death worldwide with a low five year survival rate of <1% due to its aggressive local invasion and early metastases. Thus early detection and evaluation of novel therapeutic agents is crucial for a favorable prognosis. Curcumin and Garcinol from the plants, Curcuma longa and Garcinia indica respectively have proven anti‐inflammatory, antioxidant, and anticarcinogenic activity in several cancer types. The objective of this study was to investigate the synergistic effect of these two chemopreventive dietary agents in PaCa cells. For this, PaCa cells (BxPC‐3 and Panc‐1: K‐ras wild and mutant) were treated with Curcumin (0‐50µM) and Garcinol (0 ‐ 40µM) and in combination for 24, 48 and 72hrs to examine viability, antiproliferative and apoptotic effects using MTT, Trypan Blue, cytoplasmic histone‐DNA fragment quantification and DAPI staining. The molecular mechanism was investigated by Western immunobloting, targeting different apoptotic signaling moieties. Synergistic effect of these compounds was tested using Isobologram analysis. Cell cycle phase analysis was done by Flow Cytometry. Both compounds individually and in combination significantly enhanced the anti‐proliferative and apoptotic effect in these cells relative to untreated control cells. This is first report on therapeutic effect of Garcinol on PaCa cells. Grant Funding Source Graduate Teaching Assistantship