Premium
Unsaturated fatty acids promote carotenoid bioavailability in vitro
Author(s) -
Chitchumroonchokchai Chureeporn,
Kamonpatana K.,
Ferruzzi M. G.,
Harrison E. H.,
Failla M. L.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.896.9
Subject(s) - bioavailability , chemistry , food science , carotenoid , digestion (alchemy) , biochemistry , canola , soybean oil , lipid digestion , polyunsaturated fatty acid , fatty acid , chromatography , biology , lipase , bioinformatics , enzyme
Dietary lipid is a known enhancer of carotenoid bioavailability, although the effect of type of lipid on absorption of these compounds is limited. We have investigated the effects of several common commercial lipids on bioavailability using the in vitro digestion and Caco‐2 human intestinal cells. Meals consisted of a mixed vegetable salad (3g) containing test lipid (180 mg). Micellarization and cellular uptake of β‐carotene (βC) and lycopene during small intestinal digestion was increased by lipids rich in unsaturated fatty acids: soybean oil ? olive > canola > butter. In contrast, type of lipid had minimal affect on bioaccessibility of xanthophylls. To examine the influence of lipid type on uptake and secretion of carotenoids with chylomicrons, Caco‐2 cells grown on transwell membrane were first chronically incubated with micellar mixtures of fatty acids (1.0 mM) mimicking the types and ratio of saturated to unsaturated (mono‐ + poly‐unsaturated) fatty acids (FA) present in butter (70:30), olive oil (7: 93) and soybean oil (11: 89). Then cells were exposured to micelles containing βC, LUT and a mixture of test fatty acids. Uptake and transepithelial transport of both βC and LUT were greater in cells pre‐treated with mixtures enriched in unsaturated compared to saturated FA. These results suggest that oils rich in unsaturated fatty acids promote carotenoid bioavailability. Supported by USDA NRI 2007‐02313.