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Investigating the role of cyclic AMP signaling in mushroom development in the spilt gill fungus, Schizophyllum commune
Author(s) -
Hyatt Colette E.L.,
Horton J. Stephen
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.887.2
Subject(s) - dikaryon , biology , adenylyl cyclase , microbiology and biotechnology , schizophyllum commune , mutant , gene , signal transduction , protein kinase a , genetics , mushroom , sexual reproduction , fungus , kinase , botany
The life cycle of S. commune consists of both haploid and dikaryotic phases. In normal circumstances, only the dikaryotic phase is able to produce fruiting bodies or mushrooms. Previous research in other related fungi suggests that a cAMP signalling pathway is likely to be involved in the sexual development of fruiting bodies in S. commune . The present study examines the role of members of the cAMP signaling pathway in the sexual development of this fungus . Specifically, we are studying the roles of protein kinase A and adenylyl cyclase on the formation of mushrooms by performing separate gene knockouts in S. commune . Currently, we are in the process of constructing the necessary recombinant DNA molecules necessary for use in DNA‐mediated transformation experiments. The phenotypic differences between the "knocked out" or null mutant strains generated and the wild type progenitors will be compared in order to gain insight into the role these proteins play in the life cycle of the organism. We have hypothesize that S. commune null mutants for the genes encoding protein kinase A and adenylyl cyclase will likely cause a decrease in mushroom production and/or perturbations in the normal development of these reproductive structures. Supported by a Union College IEF Grant to C.E.H.