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The effects of adrenomedullin and methylated‐adrenomedullin treatments on some antioxidative enzyme activities and malondialdehyde levels in rat liver
Author(s) -
Yurekli Muhittin,
Esrefoglu Mukaddes,
Tumer Nihal,
Dogru Ilker Mehmet,
Gul Mehmet,
Dogru Arzu,
Yýldýrým Nuran
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.881.1
Subject(s) - adrenomedullin , chemistry , superoxide dismutase , malondialdehyde , oxidative stress , glutathione peroxidase , catalase , endocrinology , antioxidant , medicine , pharmacology , biochemistry , receptor
Adrenomedullin (AdM) has many important effects such as vasorelaxant, natriuretic, proliferative/antiproliferative, regulator of adrenal hormones and ACTH secretion, antiapoptotic and antioxidant. The aim of this study is to investigate the effects of AdM and methylated‐AdM (M‐AdM) on benzo(a)pyrene (B(a)p) and heavy metals (Cd, Pb, Hg) induced oxidative stress in the rat liver. For this purpose, at first AdM was methylated. Catalase (CAT), superoxide dismutase (SOD), glutation peroxidase (GSH‐Px), and MDA levels were assessed by spectrophotometrically. Administration of M‐AdM in the Pb‐treated rats significantly increased MDA levels compared with Pb‐treated group. While AdM further decreased Pb‐induced attenuation of SOD activity, MAdM did not change. However, MAdM significantly increased SOD activity compared with AdM in Pb‐treated rats. Hg‐induced decrease of SOD activity was reversed by M‐AdM administration significantly. The administration of AdM and MAdM in B(a)p‐treated rats increased SOD, CAT and GSH‐Px activities significantly compared with B(a)p‐treated group and the increases of antioxidant enzyme activities in MAdM group more than the AdM group. These results suggest that M‐AdM may have more potent protective effect than AdM against B(a)p or heavy metal‐induced oxidative stress (Supported by The Scientific and Technological Research Council of Turkey, Project No:106T112).