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Characteristics of short gel filtration columns for rapid screening of aggregates and impurities
Author(s) -
Heijbel Anna,
Salomonson Inger,
Zuo Jinyu,
Carlsson Marianne,
Lundstrom Fredrik
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.878.1
Subject(s) - size exclusion chromatography , chromatography , filtration (mathematics) , chemistry , impurity , sample (material) , analytical chemistry (journal) , materials science , enzyme , organic chemistry , statistics , mathematics
Gel filtration (GF) is a gentle technique suitable for the study of aggregation and purity of a target protein. GF, however, is often time consuming and can also be held up due to the expense of sample and buffers. A new, small (3 ml) column format packed with Superdex(tm) 75 and Superdex 200 has been developed for rapid and reliable gel filtration enabling analyses in less than 6 min using only 4 µl of sample. Results illustrating the resolution of a standard protein mixture, sensitivity, flow rate properties and influence of system dead volumes upon resolution will be shown. The short gel filtration columns will also be compared to the longer Superdex 75 and 200 10/300 GL columns.