Premium
Differential protein expression between normal and myxomatous canine mitral valves
Author(s) -
Lacerda Carla MR,
Orton Christopher
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.857.3
Subject(s) - pathogenesis , extracellular matrix , mitral valve , heart disease , biology , pathology , heart valve , disease , differential diagnosis , shotgun proteomics , medicine , proteomics , microbiology and biotechnology , cardiology , gene , genetics
Each year, valvular heart disease accounts for over 20,000 deaths in the U.S. Myxomatous valve disease (MVD) is the most common heart valve disease in humans and dogs. MVD is pathologically identical in these species, and its pathogenesis is poorly understood. The study objectives were to 1) develop proteomic methodology suitable for analysis of extracellular matrix‐rich heart valve tissues, and 2) survey over‐ and under‐expressed proteins that could provide mechanistic clues into the pathogenesis of MVD. METHODS Normal, early‐stage and late‐stage myxomatous mitral valves from dogs were studied. A shotgun proteomic analysis of extracted proteins with iTRAQ labeling to determine differential protein quantification was employed. Proteins were classified by function and clustered according to differential expression patterns. RESULTS More than 300 proteins, with 115 of those being differentially expressed, were identified. Sample clustering showed that early‐ and late‐stage valves were closely‐related, when compared to normal valves. CONCLUSIONS Shotgun proteomic analysis of matrix‐rich canine heart valves is feasible, and should be applicable to human heart valves as well. Differential protein expression provides a basis for future investigations into the pathogenesis of canine and human MVD. Research supported by Heart to Heart foundation.