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Association of Sets of Alleles of Gene Encoding Lipoprotein Lipase Enzyme With Type 2 DM and Its Macrovascular Complications
Author(s) -
Hemimi Neveen Salah El Din,
Abd El Salam Mona Mohamed,
Abd Elwahab Mahmoud A
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.856.1
Subject(s) - lipoprotein lipase , genotype , medicine , endocrinology , allele , hindiii , type 2 diabetes , blood lipids , chemistry , gastroenterology , biology , diabetes mellitus , cholesterol , gene , biochemistry , adipose tissue , restriction enzyme
Background Lipoprotein Lipase (LPL) increases the uptake and catabolism of triglycerides rich lipoproteins (TRLs) by hydrolyzing triglycerides in chylomicrons and VLDLs and also by serving as a bridge mediating the binding of TRLs to cell surface via heparan sulphate proteoglycans leading to their uptake by a variety of receptors. Aim To detertmine whether two LPL polymorphism (LPL Hind III and LPL S447X) can be considered as predictors for DM and its macrovascular complication. Methods 176 normal individual, 42 non‐complicated type 2 diabetic patients and 78 macrovascular complicated type 2 diabetic patients were enrolled in the study. The LPL Hind III and LPL S447X genotypes were identified by RFLP‐PCR of peripheral blood DNA samples. Analysis of data was done by using SPSS program. Results Allele frequencies for H+ and S were 80.8% and 93.3% respectively in the diabetic group compared to 64.2% and 90.9% in the control group. The carriers of either (H+/H+) or (SS) variant of LPL were at high risk of developing type 2 DM (OR= 2.6; 95% CI= 1.6‐4.3 and OR= 1.4; 95% CI= 0.7‐2.7) respectively. Furthermore they were at much higher risk of developing macrovascular complications (OR= 3.7; 95% CI= 1.6‐8.2 and OR= 3.3; 95% CI =1.1‐10.0) for carrier of (H+/H+) and (SS) genotype respectively. Conclusion (H+/H+) and /or (SS) genotype were associated with Type 2 DM and its macrovasular complications. Source of research support Dubai Medical College for Girls