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Transition mutations of Hirsutella sinensis genes and expressions of Paecilomyces hepiali genes during germination and early development of Cordyceps sinensis stroma
Author(s) -
Yao Yisang,
Zhou Yanjiao,
Chen Wei,
Zhu JiaShi
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.841.1
Subject(s) - cordyceps , biology , gene , genotype , paecilomyces , stroma , germination , botany , genetics , immunohistochemistry , immunology
We reported simultaneous detections of 2 live fungi, Paecilomyces hepiali (Ph) and Hirsutella sinensis (Hs), and their genes in Cordyceps sinensis (Cs) (J Mycol Res 5:214, 2007). With Cs maturation, Hs displayed a >50% decline of its competitive colony forming ability (p<0.001), and profiles of component proteins and small organic chemicals were altered. In this study with use of Ph (ITS 5‐179) and Hs (ITS 5‐162) specific probes, Southern blotting revealed large increases in expressions of Ph and Hs genes with Cs maturation. Two species of Hs‐related Cs genes (87% identity) were identified, consisting mainly of C‐T and G‐A mutations. In premature Cs, the GC genotype expressed only in caterpillar body, while mainly AT genotype in stroma. The Ph genes and the AT genotype Hs genes appear to express synchronously during germination and early development of Cs stroma. When Cs matures, the AT genotype starts to express in caterpillar and the GC genotype in stroma. It is assumed that essential enzymes needed for the transitions between GC and AT genotypes may be provided by co‐existing fungus Ph and play key roles in assisting the anamorph‐telemorph connection of Cs.