Premium
Fibroblast Differentiation in Dermal Equivalents Affected by Tension, Age in Culture, and TGF‐β
Author(s) -
Palmer Tiffany Robin,
Vaughan Melville Bailey
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.829.1
Subject(s) - myofibroblast , transforming growth factor , fibroblast , cellular differentiation , chemistry , microbiology and biotechnology , cell culture , biology , medicine , biochemistry , fibrosis , genetics , gene
The objective of this study was to determine if tension and/or transforming growth factor beta (TGF‐β) induce stress on human fibroblasts causing the development of stress fibers and differentiation into proto‐myofibroblasts (a transitional state) and myofibroblasts and if its affect varies with cell age. The study used three different age groups of normal dermal fibroblasts including young, old, and immortal, each individually mixed with collagen in the presence or absence of a tension‐maintaining ring. Each well was given media and half of each group was given TGF‐β‐treated media. The plate was then incubated at 37°C with new media on the fifth day. After seven days the cells were fixed and one from each treatment group was selected to be stained and photographed using immunofluorescence technology. Preliminary results showed the old and young cells reacted as expected as the percentage of myofibroblasts increased with tension and TGF‐β. However, the immortal line showed little differentiation which could be attributed to their proliferation in the lattice. The development of an epidermal equivalent by adding keratinocytes will follow. Grant Funding Source NIH Idea Network of Biomedical Research Excellence P20 RR016478 ‐06