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Exploring the anti‐diabetic effect of duodenojejunal bypass: a novel mouse model
Author(s) -
Woods Michael Gregory,
Lan Zhu,
Wang Jingming,
Wang Rennian
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.825.8
Subject(s) - duodenum , jejunum , medicine , diabetes mellitus , glucose homeostasis , insulin , beta cell , endocrinology , ileum , pylorus , pancreas , gastroenterology , islet , stomach , insulin resistance
Obese patients with Type 2 diabetes who underwent bariatric surgery reverted to normal blood glucose and insulin levels and developed a dramatic increase in insulin sensitivity without continued use of diabetic medication. The mechanisms involved in this reversal are not known although the data suggest that alteration of factors such as glucagon‐like peptide‐1 (GLP‐1) produced by the upper intestinal tract is involved. Currently, no studies have focused on a non‐diabetic DJB animal model to determine its importance of the duodenum and proximal jejunum in maintaining normal glucose homeostasis. Normal C57BL/6J mice underwent duodenojejunal bypass (DJB), a surgery in which the distal end of the jejunum was connected to a small portion of the duodenum just distal to pylorus and bilopancreatic secretions where directed to the distal jejunum. Normal‐fed mice undergoing DJB display diabetic‐like pancreatic morphology. These mice showed a decrease in the percent area of beta‐cells (33%) as well as a decrease in beta‐cell proliferation (50%) compared to sham‐operated controls. However, surviving beta‐cells showed similar levels of pancreatic duodenal homeobox‐1 (Pdx‐1), GLUT‐2 expression, and similar beta‐cell size between DJB and sham‐operated groups. These results suggest the importance of the duodenum in maintaining an anti‐diabetic state in the normal mouse. Funded by Lawson Health Research Institute. Grant Funding Source Lawson Health Research Institute