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Simultaneous integration of biosensor and EEG measurements in freely moving rodents
Author(s) -
Johnson David A.,
Aillon Daniel V.,
Naylor Erik,
Harmon Hans,
Wilson George S.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.818.10
Subject(s) - electroencephalography , wakefulness , sleep (system call) , sleep deprivation , medicine , neuroscience , glutamate receptor , biomedical engineering , endocrinology , anesthesia , chemistry , circadian rhythm , psychology , computer science , receptor , operating system
Second by second measurement of neurochemicals such as glutamate, glucose and lactate in the brain using electrochemical biosensors is a powerful tool that has been used to profile concentration changes due to pharmacological and behavioral interactions. Now this ability to measure metabolic changes in targeted regions of the brain has been integrated with a very low‐noise, turn‐key electroencephalograph (EEG) recording system. As an example of this system's strength, male C57 mice were simultaneously implanted with an intracerebral guide cannula targeting the prefrontal cortex and electrodes to measure cortical EEG. Real‐time, second by second measurements of glucose levels and EEG activity were recorded over a 24 hour period. Results showed that glucose levels in mice transitioning from waking to slow‐wave sleep rose an average of 116 ± 27.0 uM (n =57 episodes). Conversely, glucose levels in mice transitioning from sleep to wakefulness resulted in an average decrease of 121 ± 27.0 uM glucose. (n=54 episodes). Additional data will be presented demonstrating the system being used to monitor changes of neurotransmitters during normal sleep and periods of sleep deprivation. Funding through NIH SBIR Grant #5R44MH076318‐03.