Premium
Porous PEGDA hydrogels promote hMSC survival and eventual differentiatability
Author(s) -
Gemeinhart Richard A,
Keskar Vandana
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.817.3
Subject(s) - self healing hydrogels , ethylene glycol , scaffold , microbiology and biotechnology , tissue engineering , chemistry , chondrocyte , mesenchymal stem cell , cell , biophysics , biomedical engineering , biochemistry , biology , in vitro , polymer chemistry , medicine , organic chemistry
Superporous poly(ethylene glycol) diacrylate (PEGDA) hydrogel scaffolds have the potential to overcome the problems of difficulty seeding cells into the scaffold. The interconnected pore network of the superporous hydrogels (SPH) not only allows cells to attach to the interior porous structure but also facilitates nutrient exchange within cells in the construct. We have shown that cells hMSC can rapidly and uniformly loaded in to the scaffold, can survive for up to a month, and can mineralize the tissue (Tissue Engineering, in press). Very interestingly, hMSCS, but not HUVECs, adhere within the porous network in an RGD‐peptide independent manner. In addition to this ability, hMSCs retain ability to differentiate within the porous network to cell types that appear as chondrocyte, osteoblast, and adipocyte upon stimulation with appropriate medium. MSCs treated with a specific inducing media or untreated controls do not exhibit markers for the cell types other than the appropriate type of the inducing medium, or no cell markers for untreated control cells.