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Smooth Muscle Cell Seeding on Decellularized Porcine Saphenous Vein Scaffolds –A Step Towards Functional Tissue Engineered Blood Vessels
Author(s) -
Machingal Masood A.,
Merritt Heather P.M.,
Neff Luke P,
Van Dyke Mark,
Soker Shay,
Geary Randolph,
Christ George J.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.817.2
Subject(s) - decellularization , biomedical engineering , scaffold , pulsatile flow , bioreactor , chemistry , tissue engineering , masson's trichrome stain , dapi , anatomy , staining , medicine , pathology , cardiology , apoptosis , biochemistry , organic chemistry
The need for improved autologous tissue engineered blood vessels (TBEV) for vascular replacement is unequivocal. To this end, the over‐riding goal of our vascular research program is to develop TEBV that more closely approximate native vasculature. To this end, we have utilized an approach that combines dual‐seeding of endothelial and smooth muscle cells on decellularized arterial scaffolds (DAS) followed by bioreactor preconditioning. A major barrier to this approach is the limited migration of SMC into the medial layer of the DAS. To overcome this limitation, the current studies explored the potential of decellularized venous scaffolds (DVS). DVS were statically seeded with SMC for 2 hours and preconditioned in a bioreactor with either pulsatile flow (60 bpm /80‐120 mm Hg) or steady flow (100 mm Hg) for two weeks. The effect of cyclic strain preconditioning on attachment, proliferation and migration of SMCs was evaluated with H&E, Trichrome and DAPI. Our preliminary data suggest that seeded SMC adhere to and proliferate on DVS. We also demonstrated that cyclic preconditioning using pulsatile flow improves SMC migration and integration/ingrowth into the medial layer of the scaffold. Although further study is required, these initial data are encouraging and indicate that in vitro conditioning of DVS may produce TEBV that more closely mimic the architecture and physiological characteristics of native arteries.