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Solid‐phase tissue electrophoresis enhances sodium dodecyl sulfate‐based decellularization of xenogeneic bioscaffolds
Author(s) -
Arai Shiori,
Orton Christopher
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.817.1
Subject(s) - decellularization , sodium dodecyl sulfate , chemistry , gel electrophoresis , agarose , tissue engineering , antigen , microbiology and biotechnology , antigenicity , chromatography , biochemistry , biomedical engineering , biology , extracellular matrix , immunology , medicine
Treatment of xenogeneic bioscaffolds with ionic detergents does not completely remove soluble protein antigens from candidate xenogeneic scaffolds for tissue engineering. The study objective was to determine if solid‐phase tissue electrophoresis enhances removal of antigenic proteins from candidate bioscaffolds. METHODS Porcine aortic valve conduit (PAV) was subjected to hypotonic lysis and then treated with 0.25% or 1% sodium dodecyl sulfate (SDS) overnight Tissues were mounted in 2% agarose gel and subjected 0 (sham), 60, or 120 V for 4 h. After treatment, soluble proteins were extracted from tissues and assayed for antigenicity by immunoblot analysis with rabbit immune serum. RESULTS Solid‐phase tissue electrophoresis enhanced removal of protein antigens from PAV compared to untreated (UT) or sham treated controls (figure). CONCLUSION Solid‐phase tissue electrophoresis enhances SDS‐based bioscaffold decellularization. Supported by NIH HL081107.