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Effect of forced exercise on differential expression of caveolin isoforms in MCAo‐operated rat tissues
Author(s) -
Park Sookyoung,
Won Jinyoung,
Lee Youngjeon,
Hong Yonggeun
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.811.3
Subject(s) - caveolin 3 , myostatin , medicine , endocrinology , skeletal muscle , endurance training , caveolin , caveolae , muscle hypertrophy , gene isoform , biology , microbiology and biotechnology , signal transduction , biochemistry , gene
Endurance exercise has many health benefits, including improvements in muscle metabolism, cardiovascular function, and increased exercise tolerance, also induce mitochondrial biogenesis in skeletal muscle. Exercise training can improve strength and cause adaptations in the skeletal muscle and nervous systems. Caveolins are integral membrane protein, which play a role in essential cellular functions. Especially, caveolin‐1 was reported to inhibit the activation of the type I receptor for TGF‐β1, which induces growth arrest in non‐muscle cells. Cav‐3 normally suppresses the myostatin‐mediated signal, thereby preventing muscular atrophy, Thus, this study was to reveal that the changes of expression of caveolins after forced exercise using by treadmill in muscular and non‐muscular tissues. Male(n=16) and female(n=16) Wistar rat were introduced to forced exercise with 25m/min speed for 30min, 3 times/day each time points at 07:00, 15:00 and 23:00. Then, the tissues were homogenized, total RNA extracted from gastrocnemius (fast‐twitched), soleus (slow‐twitched) muscle, and non‐muscle tissue of female rat using RNeasy kit (Qiagen, Germany) for further analysis. Stable expression of caveolin‐3 was detected even in all tissues applied of exercise group compared to control. Strikingly, caveolin‐1 and ‐2 were dramatically changed at expression level in the tissues of exercise group. Also, caveolin isoforms was shown as tissue specific differential expression. Keywords: Caveolae, caveolin, forced exercise, muscle type. Supported by BioGreen 21 Program (#20070401034006) from Rural development Administration, South Korea.