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Altered expression of paternally imprinted genes is associated with fetoplacental demise in the BPH/5 model of pre‐eclampsia (PE)
Author(s) -
Woods Ashley Kathryn,
Zhou Yi,
Sharma Ram V,
Davisson Robin L
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.807.7
Subject(s) - genomic imprinting , placentation , imprinting (psychology) , biology , gene , placenta , andrology , fetus , microarray analysis techniques , microarray , placental growth factor , endocrinology , insulin like growth factor 2 , medicine , gene expression , pregnancy , genetics , preeclampsia , dna methylation
Pregnancies in BPH/5, a mouse model of PE, are characterized by abnormal placentation and fetal demise starting early in gestation. Paternally imprinted genes, often found in the placenta, have been implicated as regulators of placental and fetal growth. We hypothesized that paternally imprinted genes would be dysregulated in BPH/5. Gene expression microarray studies were carried out on BPH/5 fetoplacental units sonographically scored and categorized into three increasingly severe phenotypic classes based on qualitative and quantitative parameters. The three classes showed a total of 697 dysregulated genes. Three paternally imprinted genes were dysregulated in BPH/5 and were verified with real time PCR. These include: insulin growth factor 2 (Igf2) which was decreased in Class II and III fetoplacental units(0.32 ± 0.13 and 0.26±0.17 fold vs C57, n=3 p<0.05), delta‐like 1 (Dlk‐1) which was decreased in Class II and III (0.08 ± 0.04 and 0.19 ± 0.13 fold vs C57, n=3 p<0.05), and paternally expressed gene 3 (Peg3) which was decreased in Class II (0.22 ± 0.09 vs C57, n=3 p<0.05). Recent studies using knockout mice show that these paternally imprinted genes regulate placental size and depth, suggesting that these genes may be linked to fetoplacental abnormalities in our model of PE.