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Regulation of ciliary beat frequency by sex hormones in guinea pig Fallopian tube: interactions between estrogen and progesterone
Author(s) -
Nakahari Takashi,
Nishimura Ayumi
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.807.13
Subject(s) - mifepristone , endocrinology , medicine , fallopian tube , estrogen , estradiol benzoate , chemistry , ovariectomized rat , biology , pregnancy , anatomy , genetics
Fallopian tube CBF was measured using a microscope connecting a high‐speed camera (500 Hz). β‐Estradiol‐benzoate (βE2‐benz) and medroxy‐progesterone (m‐Prog) were administered by subcutaneous injection for 1.5 days. βE2‐benz changed CBF in a dose dependent manner; a low dose (2 mg/day) increased CBF (14.5 Hz), whereas a high dose (20 mg/day) decreased it (10.83 Hz). An inhibitor of βE2 (2 mg/day, ICI‐182,780) maintained CBF at 12 Hz, and the further addition of βE2‐benz did not change CBF. m‐Prog (4 mg/day) decreased CBF (12.8 Hz), and an inhibitor of progesterone (50mg/day, mifepristone) maintained CBF at 15 Hz, and the further addition of m‐Prog did not change CBF. The addition of both inhibitors increased CBF to 17.4 Hz, and then, the addition of m‐Prog, instead of mifepristone, decreased it to 11 Hz, however, the further addition of βE2‐benz, instead of ICI 182,780, increased to 17.5 Hz. Thus, βE2‐benz inhibited the m‐Prog action. Moreover, m‐Prog shifted the CBF dose response curve of βE2‐benz to the right, suggesting m‐Prog decreased the CBF sensitivity to βE2‐benz. In conclusion, Fallopian tube CBF was regulated by interactions between estrogen and progesterone. The experiments were approved by the Animal Research Committee of our College.