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Expression of endothelin, endothelin type A receptor, isoforms of nitric oxide synthase, and angiotensin receptors in endothelin type B receptor deficient mice
Author(s) -
RettigZimmermann Juliane,
Schildroth Janice,
Kalk Philipp,
Steege Andreas,
Fähling Michael,
Persson Pontus Börje,
Hocher Berthold,
Patzak Andreas
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.804.5
Subject(s) - medicine , endocrinology , endothelin receptor , receptor , enos , endothelin 1 , angiotensin ii , kidney , renin–angiotensin system , nitric oxide synthase , chemistry , messenger rna , biology , nitric oxide , blood pressure , biochemistry , gene
Endothelin‐1 (ET‐1) is involved in the control of renal vessel contractions by acting on endothelin type A (ETA) and type B (ETB) receptors. Several studies indicate an interaction of ET‐1 with the angiotensin system; which includes modulation of receptor expression. The aim of this study was to determine the influence of ETB receptor deficiency on the expression of ET‐1, ETA receptors, nitric oxide synthases (NOS) as well as angiotensin receptors (AT) in preglomerular vessels and kidneys of ETB(‐/‐) mice. ETA mRNA expression, determined by real time PCR, was decreased in preglomerular vessels compared to wild type mice (ETB(+/+)). ETA mRNA and protein were also diminished in whole kidney preparations in ETB(‐/‐) mice. The endothelial NOS (eNOS) mRNA was less expressed in preglomerular vessels. No significant differences were found for mRNA expression of eNOS, nNOS, and iNOS in whole kidney preparations. AT 1 and AT 2 receptor mRNA in the kidney did not differ between ETB(‐/‐) and ETB(+/+). As shown by western blotting analysis the ET‐1 protein was stronger expressed in the kidneys of ETB(‐/‐) The results of ET‐1 and ETA expression confirm earlier findings of a clearance function of the ETB receptor and modulation of ETA expression by ET‐1. Moreover, ET receptor expression/function seems to influence eNOS mRNA expression in renal arterioles.