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Quantitative trait loci for exercise capacity and response to training in FVB/NJ and C57BL/6J mice
Author(s) -
Massett Michael P,
Berk Bradford C
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.801.6
Subject(s) - quantitative trait locus , treadmill , biology , inbred strain , genetics , zoology , gene , physiology
The genetic factors determining the magnitude of the response to exercise training are poorly understood. The aim of this study was to identify quantitative trait loci (QTL) associated with adaptation to exercise training in mice. Mice completed an exercise performance test before and after a 4‐week treadmill running program and changes in exercise capacity, expressed as work (kg‐m), were calculated. The change in work with exercise training was significantly different between inbred C57BL/6J (B6) and FVB/NJ (FVB) mice (0.34±0.12 vs. 1.78±0.17 kg‐m, P<0.05). FVBxB6 F 1 mice had an intermediate phenotype (1.21±0.35). In F 2 mice (n=188), changes in work ranged from ‐1.67 to +4.55 kg‐m. All F 2 mice were genotyped at 20 cM intervals using 103 SNPs and genome scans were performed. Suggestive QTL (P=0.63) for changes in work were found on chromosomes 3, 11, and 14. In addition, significant QTL (P<0.05) were identified for pre‐ and post‐training work. QTL for pre‐training work were located on chromosomes 8 (peak at 60.1 cM, 4.47 LOD), 14 (15.3 cM, 4.16 LOD), and 19 (40.6 cM, 3.68 LOD). QTL for post‐training work were located on chromosomes 3 (72.2 cM, 4.83 LOD) and 14 (21.3 cM, 4.88 LOD). These data indicate that one or more QTL determine exercise capacity and training responses in mice. Furthermore, these data suggest that the genes that determine pre‐training work and training responses may differ. Supported by AHA SDG and HL‐085918 (MPM) and HL‐62826 (BCB).