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Regulation of V‐ATPase, NBCe1‐A and NBCe1‐B in hippocampus
Author(s) -
Martin Henno,
Oehlke Oliver,
Roussa Eleni
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.799.3
Subject(s) - extracellular , intracellular ph , endocrinology , intracellular , medicine , chemistry , microbiology and biotechnology , cytosol , biology , biochemistry , enzyme
The electrogenic sodium‐bicarbonate‐cotransporter (NBCe1) is a key player in regulation of intracellular pH in several cell types. In the CNS however, modes of intracellular pH regulation reveal considerable differences, compared to other tissues, because neuronal activity is accompanied by rapid pH shifts. Here we have investigated expression and regulation of V‐ATPase, and N‐terminal variants of NBCe1 in mouse brain. V‐ATPase, NBCe1‐A and NBCE1‐B expression was detected in hippocampus, cerebral cortex, olfactory bulb and cerebellum. However, NBCe1 N‐terminal variants revealed distinct cellular distribution in mouse brain and showed differential activity‐dependent regulation in vitro. After induction of extracellular acidosis or alkalosis gene expression and protein expression of V‐ATPase, NBCe1‐A and NBCe1‐B in hippocampal cultures were not changed. However, acute extracellular acidosis resulted to V‐ATPase and NBCe1‐A redistribution from the cytosol towards the plasma membrane. The results suggest redistribution of acid‐base transporters as main regulation mechanismus during acid‐base disturbances. Moreover, NBCe1 may be involved in neuronal modulation, and pH regulation during neuronal activity.